AB0160 GUT MICROBES AND SYSTEMIC SCLEROSIS: CAUSE OR CONSEQUENCE?

Background There is growing evidence supporting an association between gut microbiota and the risk of systemic sclerosis (SSc)[1]. However, causal relationship SSc remains ambiguous. Objectives A two-sample Mendelian randomization (MR) analysis was performed to reveal SSc. Methods We obtained data on intestinal flora from MR-base website [2] 218,499 samples for IEU database a MR analysis. Inverse variance weighting, MR-Egger, weighted median were used examine Then series sensitivity analyses verify robustness results. Finally, bacteria which appeared causality with subjected reverse assess possibility causality. Results variance-weighted estimates indicated that genus Collinsella (odds ratio(OR) = 8.982, 95% confidence interval(CI): 1.571-51.366，P 0.014), phylum Actinobacteria (OR 5.746, CI: 1.247-26.491，P 0.025), Ruminococcaceae 5.500, 1.477-20.477，P 0.011) had effect ( Table 1 ). According results analysis, no significant flora. No heterogeneity or horizontal pleiotropy instrumental variables found. Conclusion The present study revealed three microflora genera SSc, providing new insights into mechanisms microbiota-mediated scleroderma development. References [1]Lemos, M.P.C., et al., Dysbiosis Gut Microbiota Modulation in Systemic Sclerosis. J Clin Rheumatol, 2022. 28 (2): p. e568-e573. [2]Kurilshikov, A. C. Medina-Gomez, Large-scale identify host factors influencing human microbiome composition. 2021. 53 156-165. 1. N SNPs Cochran’s Q statistic(p-value) Odds ratio CI p-value Genus 9 3.853 (0.870) 8.982 (1.571，51.366) 0.014 Egger 3.545 (0.8301) 1.515 (0.002，1029.541) 0.904 Weighted 14.103 (1.466，135.692) 0.022 Phylum 15 17.720 (0.220) 5.746 (1.247,26.491) 0.025 16.880 (0.205) 79.147 (0.110, 57116.960) 0.216 2.109 (0.275,16.168) 0.448 12 6.317 (0.851) 5.500 (1.477,20.477) 0.011 6.183 (0.800) 2.552 (0.034,191.160) 0.680 5.698 (1.009,32.173) 0.052 sclerosis; MR, randomization; CI, interval Acknowledgements: NIL. Disclosure Interests None Declared.